The capillaries (Fig 1F) could also be seen. In

The renal cortex is characterized by the presence of multiple renal
corpuscles and renal tubules that include proximal, distal convoluted tubules,
and collecting tubules, renal glomeruli were formed
of lobulated tufts of capillaries. They were seen surrounded by Bowman’s
capsule. The parietal layer of Bowman’s capsule was lined by a single layer of
simple squamous epithelium. Between the two layers of Bowman’s capsule, a
patent urinary space was seen (Fig. 1A & 1B).  In this study, the renal
cortex of the CCl4 treated group showed congestion of blood
capillaries, many degenerated proximal and distal convoluted tubules (PCT) with
dilated tubular lumens, vaculated tubular epithelium and flattened tubular
epithelium (Fig. 1C). The renal glomerulus showed hypercellularity with dilated
luminal spaces and infiltration with inflammatory cells (Fig. 1D).BM-MSCs-treated rats showed enhancement of glomerular
structure which surrounded by Bowman’s capsule, filtration space. The proximal
convoluted tubules were most propably normal with preserved brush border. Most
renal tubules were seen almost similar to the control group (Fig. 1E). While
the recovery group showed obliterating the Bowman’s space. Congestion of
peritubular blood capillaries (Fig 1F) could also be seen. In the present study, Mallory’s trichrome stained sections revealed
the normal distribution of collagen fibers around glomerulus and renal tubules
within the renal cortex in control rats (Fig. 2A & 2B). In contrast to the
CCl4 treated rats, showed increased amount of collagen fibers in the
glomerulus and interstitium within the renal cortex (Fig. 2C). BM-MSCs-treated
rats showed the same finding compared to rats (Fig. 2D). Moderately increased
amount of collagen fibers around the glomerulus and interstitium were observed
in the recovery group (Fig. 2E).In the present investigation,
the greatest actin deposition was found in CCl4-treated
rats in epithelial tubular cells and in the renal glomeruli due to strong
positive reaction of ?-SMA (Fig. 3B),
followed by the withdrawal group which showed negative reaction in glomerulus
and positive reaction in renal tubules (Fig. 3D). While
the control and BM-MSCs-treated groups revealed a negative reaction in glomerulus,
renal tubules or interstitium, positive reaction is only seen around blood
capillaries (Fig. 3A & 3C). In this study, ultrastructural
examination of the control group showed that the PCTs had a normal structure.
They were lined with cuboidal cells with regular microvilli, numerous elongated
mitochondria enclosed in the regular basal enfolding (Fig. 4A). whereas those
of the CCl4
treated rats showed apical shortened brush border and dark nuclei (N) with
condensed chromatin. Numerous lysosomes and endosomes were also observed in their
cytoplasm (Figs. 4B & 4D). In contrast, examination of PCT of mesenchymal stem cell
(MSCs) treated group revealed evidence of histological rebuilding of typical
renal structure with regular
microvilli compared with the control group (Fig. 4C).The DCTs (Distal convoluted
tubules) of the control group showed low cuboidal cells with euchromatic
nuclei, regular basal infoldings, and elongated mitochondria in between. Short
apical plasma membrane microprojection can be observed (Fig. 5A). In contrast,
the DCTs of CCl4 treated rats showed disoriented basal
infoldings with some swollen disrupted mitochondria in between, dilated
enfolding of the basolateral plasma membrane. Also shrunken lumen with loss of
apical plasma membrane microprojection can be observed (Fig. 5B). The same findings were observed in the DCTs
of recovery group; however, afew area of cytoplasm were rarefied (Fig. 5D). The
DCTs of BM-MSCs-treated rats had preserved tubular cells ultrastructure in this
group compared to control group (Fig. 5C).The renal
corpuscle of control rats revealed normal ultrastructure (Figs. 6A &6B). The podocyte of
the internal layer of Bowman’s capsule had central euchromatic nucleus surrounded by cytoplasm. This podocyte had thick primary
cytoplasmic processes that expanded and subdivided into many thin auxiliary
cytoplasmic processes on the external part of the basal lamina of numerous
glomerular capillary loops. Slit diaphragms of the capillary loops were seen
between the secondary cytoplasmic processes. The glomerular capillary lined
by discontinuous endothelial cells and contains RBCs. A thin capsular space was seen between the podocyte and the basal
lamina of the capillary tuft. On the other hand the renal corpuscle in CCl4-treated
rats revealed congestion of the glomerular capillary with fusion of the
secondary foot processes with loss of slit diaphragms and widening of the capsular space. Also the renal
corpuscle showed numerous mesangial cells in-between
the capillary loops (Fig. 6C & 6D). The same histological finding
were seen in the recovery group (Fig. 7C & 7D)

The renal
corpuscle of BM-MSCs-treated
rats showed more or less normal podocyte with euchromatic nucleus. This
podocyte had primary thick foot process and numerous secondary foot processes
on basement membane of glomerular capillary compared to control group (Fig. 7A
& 7B).

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