Present is a vegetative method for multiplication of plants.

Present study deals with
the study of some Solanaceous plants from Chandigarh and its adjoining areas
for their medicinal importance. According to the survey, a total of genera and
species are present and amongst which five genera namely Datura, Solanum,
Cestrum, Nicotiana and Physalis were selected. Two species of Datura namely Datura inoxia, Datura metel, two of solanum namely Solanum torvum and Solanum verbascifolium , two of cestrum
namely Cestrum nocturnum and Cestrum diurnum, one each of Nicotiana namely Nicotiana plumbaginifolia and Physalis
minima were selected for the present study.

In vitro cell & tissue
culture methodology is envisaged as a mean for germplasm conservation to ensure
the survival of endangered plant species, rapid mass propagation for large
scale revegetation and for genetic manipulation studies.  Combination of in vitro propagation technique
(Fay, 1992) and cryopreservation may help in the conservation of biodiversity
in general and locally used medicinal plants in particular.  In vitro propagation holds tremendous
potential for the production of high-quality plant based medicine. In vitro
production of secondary metabolites in plant cell suspension cultures and
callus has been reported from various medicinal plants.  Plant micropropagation is the technique of
growing plant cells, tissues and organs in an artificially prepared media (static
or liquid) under aseptic conditions.  It
is a vegetative method for multiplication of plants.  It has advanced the knowledge of fundamental
botany, agriculture, phytopathology, industrial production of plant metabolites
and transgenic plants. In vitro propagation is a best technique to increase the
population of medicinally important plants which do not give seeds or whose
seeds have a low germination capacity. Through micropropagation, such plants
will be made available throughout the year. Tissue culture helps to produce the
uniform clones from highly heterozygous plants and conservation of genetic
resources of species and threatened plants. 
Micropropagation has many advantages over conventional methods of vegetative
propagation, which suffer from several limitations. With micropropagation, the
multiplication rate is greatly increased. The micropropagation has the
advantage of facilitating the production of uniform and healthy plants, as well
as to reduce propagation time. Further, micropropagation techniques do not
depend on the climatic conditions and are especially useful in species that
have recalcitrant seeds which rapidly loose their viability.  It is the case of several tropical perennial
species.  It is important for long term
conservation efforts to preserve the biodiversity of endangered species and
ecosystems (Siddique et al 2007). It also permits the production of pathogen
free material. Micropropagation of various plant species including many
medicinal plants has been using successfully by many workers. Medicinal plants
are of great interest to the researchers in the field of biotechnology as most
of the drug industries depend, in part, or plants for the production of
pharmaceuticals compounds

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